Elucidation of the presence and location of t-Boc protecting groups in amines and dipeptides using on-column H/D exchange HPLC/ESI/MS was written by Wolf, Christian;Villalobos, Cristina N.;Cummings, Paul G.;Kennedy-Gabb, Sonya;Olsen, Mark A.;Trescher, Gudrun. And the article was included in Journal of the American Society for Mass Spectrometry in 2005.Name: tert-Butyl methyl(6-methylpyridin-2-yl)carbamate This article mentions the following:
High performance liquid chromatog./mass spectrometry (HPLC/MS) has become a widely used technique for routine anal. of pharmaceutical compounds The constant search for new drugs requires the development of time-efficient methods that can be employed in high-throughput screening of combinatorial libraries of a variety of compounds, including amines and peptides. Conventional HPLC/MS is a powerful technique that can easily be automated and is suitable for comprehensive screening purposes. However, the unequivocal determination of the presence and location of important carbamoyl protecting groups of amines is often elusive because of their inherent instability under MS conditions. In this study, the use of on-column H/D exchange HPLC/ESI/MS for structure elucidation of t-Boc protecting groups which can often not be detected by MS because of facile McLafferty rearrangement has been examined We demonstrate that employing a deuterated mobile phase in HPLC/MS anal. provides a convenient tool for the determination of the absence or presence of t-Boc protecting groups in amines and peptides. In the experiment, the researchers used many compounds, for example, tert-Butyl methyl(6-methylpyridin-2-yl)carbamate (cas: 205676-84-2Name: tert-Butyl methyl(6-methylpyridin-2-yl)carbamate).
tert-Butyl methyl(6-methylpyridin-2-yl)carbamate (cas: 205676-84-2) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Pyridine, its benzo and pyridine-based compounds play diverse roles in organic chemistry. Pyridine-based materials are valued for their optical and physical properties as well as their medical potential. Name: tert-Butyl methyl(6-methylpyridin-2-yl)carbamate