Category: 500-22-1

Computed Properties of C6H5NO. Sepahvand, H; Ghasemi, E; Sharbati, M; Mohammadi, MS; Pirlar, MA; Shahverdizadeh, GH in [Sepahvand, Heshmatollah; Ghasemi, Elnaz; Shahverdizadeh, Gholam Hossein] Islamic Azad Univ, Dept Chem, Tabriz Branch, Tabriz, Iran; [Sharbati, Mohammad] Univ Tehran, Sch Met & Mat Engn, Coll Engn, Tehran, Iran; [Mohammadi, Melika Sadat; Pirlar, Maghsoud Arshadi] Shahid Beheshti Univ, Dept Phys, Tehran, Iran published The magnetic graphene oxide/NHC catalyzed aerobic direct amidation and cross-dehydrogenative coupling of aldehydes in 2019.0, Cited 53.0. The Name is 3-Pyridinecarboxaldehyde. Through research, I have a further understanding and discovery of 500-22-1.

In this paper, the synthesis of a novel imidazolium based N-heterocyclic carbene (NHC) containing imidazopyridine substitutes using a three-component reaction is described. This compound was immobilized on magnetic graphene oxide (GO) to make a heterogeneous catalyst for the direct aerobic amidation of benzaldehyde derivatives under solvent-free conditions, as well as the intra- and intermolecular cross dehydrogenative coupling of aldehydes. In addition, the catalytic activity of the NHC catalyst was homogeneously studied in the presence of Fe3O4 nanoparticles under the same conditions, and showed good activity with lower yields than the heterogeneous catalyst.

Computed Properties of C6H5NO. Bye, fridends, I hope you can learn more about C6H5NO, If you have any questions, you can browse other blog as well. See you lster.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

Authors Garai, S; Kulkarni, PM; Schaffer, PC; Leo, LM; Brandt, AL; Zagzoog, A; Black, T; Lin, XY; Hurst, DP; Janero, DR; Abood, ME; Zimmowitch, A; Straiker, A; Pertwee, RG; Kelly, M; Szczesniak, AM; Denovan-Wright, EM; Mackie, K; Hohmann, AG; Reggio, PH; Laprairie, RB; Thakur, GA in AMER CHEMICAL SOC published article about ENDOCANNABINOID SYSTEM; INTRAOCULAR-PRESSURE; NEUROPATHIC PAIN; SIGNALING SYSTEM; CB1; PROTEIN; STRATEGIES; DESIGN; SELECTIVITY; AGONISM in [Garai, Sumanta; Kulkarni, Pushkar M.; Schaffer, Peter C.; Thakur, Ganesh A.] Northeastern Univ, Bouve Coll Hlth Sci, Sch Pharm, Dept Pharmaceut Sci, Boston, MA 02115 USA; [Brandt, Asher L.; Zagzoog, Ayat; Black, Tallan; Laprairie, Robert B.] Univ Saskatchewan, Coll Pharm & Nutr, 104 Clin Pl, Saskatoon, SK S7N 2Z4, Canada; [Leo, Luciana M.; Abood, Mary E.] Temple Univ, Lewis Katz Sch Med, Ctr Subst Abuse Res, Philadelphia, PA 19140 USA; [Hurst, Dow P.; Reggio, Patricia H.] Univ North Carolina Greensboro, Ctr Drug Discovery, Greensboro, NC 27402 USA; [Janero, David R.] Northeastern Univ, Bouve Coll Hlth Sci, Coll Sci & Hlth Sci Entrepreneurs, Dept Chem & Chem Biol,Dept Pharmaceut Sci, Boston, MA 02115 USA; [Lin, Xiaoyan; Zimmowitch, Anaelle; Straiker, Alex; Mackie, Ken; Hohmann, Andrea G.] Indiana Univ, Program Neurosci Psychol & Brain Sci, Bloomington, IN 47405 USA; [Lin, Xiaoyan; Zimmowitch, Anaelle; Straiker, Alex; Mackie, Ken; Hohmann, Andrea G.] Indiana Univ, Gill Ctr Biomol Sci, Bloomington, IN 47405 USA; [Kelly, Melanie; Szczesniak, Anna-Maria; Denovan-Wright, Eileen M.; Laprairie, Robert B.] Dalhousie Univ, Fac Med, Dept Pharmacol, 5850 Coll St, Halifax, NS B3H 4R2, Canada; [Pertwee, Roger G.] Univ Aberdeen, Inst Med Sci, Sch Med Med Sci & Nutr, Aberdeen AB25 2ZD, Scotland in 2020.0, Cited 69.0. HPLC of Formula: C6H5NO. The Name is 3-Pyridinecarboxaldehyde. Through research, I have a further understanding and discovery of 500-22-1

Cannabinoid 1 receptor (CB1R) allosteric ligands hold a far-reaching therapeutic promise. We report the application of fluoro- and nitrogen-walk approaches to enhance the drug-like properties of GAT211, a prototype CB1R allosteric agonist-positive allosteric modulator (ago-PAM). Several analogs exhibited improved functional potency (cAMP, beta-arrestin 2), metabolic stability, and aqueous solubility. Two key analogs, GAT591 (6r) and GAT593 (6s), exhibited augmented allosteric-agonist and PAM activities in neuronal cultures, improved metabolic stability, and enhanced orthosteric agonist binding (CP55,940). Both analogs also exhibited good analgesic potency in the CFA inflammatory-pain model with longer duration of action over GAT211 while being devoid of adverse cannabimimetic effects. Another analog, GAT592 (9j), exhibited moderate ago-PAM potency and improved aqueous solubility with therapeutic reduction of intraocular pressure in murine glaucoma models. The SAR findings and the enhanced allosteric activity in this class of allosteric modulators were accounted for in our recently developed computational model for CB1R allosteric activation and positive allosteric modulation. [GRAPHICS] .

HPLC of Formula: C6H5NO. Bye, fridends, I hope you can learn more about C6H5NO, If you have any questions, you can browse other blog as well. See you lster.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

I found the field of Chemistry very interesting. Saw the article Distinctive reactivity of N-benzylidene-[1,1 ‘-biphenyl]-2-amines under photoredox conditions published in 2020. Application In Synthesis of 3-Pyridinecarboxaldehyde, Reprint Addresses Cho, EJ (corresponding author), Chung Ang Univ, Dept Chem, 84 Heukseok Ro, Seoul 06974, South Korea.. The CAS is 500-22-1. Through research, I have a further understanding and discovery of 3-Pyridinecarboxaldehyde

A simple photocatalytic method was developed for the synthesis of unsymmetrical 1,2-diamines by the unprecedented reductive coupling of N-benzylidene-[1,1′-biphenyl]-2-amines with an aliphatic amine. The presence of a phenyl substituent in the aniline moiety of the substrate was critical for the reactivity. The reaction proceeded via radical-radical cross-coupling of alpha-amino radicals generated by proton-coupled single-electron transfer in the presence of an Ir photocatalyst. On the other hand, symmetrical 1,2-diamines were selectively produced from the same starting materials by the judicious choice of the reaction conditions, showcasing the distinct reactivity of N-benzylidene-[1,1′-biphenyl]-2-amines. The developed method can be employed for the synthesis of various bulky vicinal diamines, which are potential ligands in stereoselective synthesis.

Application In Synthesis of 3-Pyridinecarboxaldehyde. Welcome to talk about 500-22-1, If you have any questions, you can contact Tambe, SD; Min, KH; Iqbal, N; Cho, EJ or send Email.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

HPLC of Formula: C6H5NO. Recently I am researching about BIOLOGICAL EVALUATION; 1,4-PENTADIEN-3-ONE DERIVATIVES; VIRUS; CURCUMIN; BEARING; 3D-QSAR, Saw an article supported by the National Key Research and Development Program of China [2017YFD0200506]; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21462012, 21502032]; Special Fund for Outstanding Scientific and Technological Candidates of Guizhou Province [201535]. Published in BMC in LONDON ,Authors: Chen, LJ; Wang, XB; Tang, X; Xia, RJ; Guo, T; Zhang, C; Li, XY; Xue, W. The CAS is 500-22-1. Through research, I have a further understanding and discovery of 3-Pyridinecarboxaldehyde

Background: penta-1,4-diene-3-one oxime ether and quinazolin-4(3H)-one derivatives possess favorable agricultural activities. Aiming to discover novel molecules with highly-efficient agricultural activities, a series of penta-1,4-diene-3-one oxime ether derivatives containing a quinazolin-4(3H)-one scaffold were synthesized and evaluated for their antiviral activities. Result: Antiviral bioassays indicated that some title compounds exhibited significant antiviral activity against tobacco mosaic virus (TMV). In particular, compounds 8c, 8j and 8k possessed appreciable curative activities against TMV in vivo, with half-maximal effective concentration (EC50) values of 138.5, 132.9 and 125.6 mu g/mL, respectively, which are better than that of ningnanmycin (207.3 mu g/mL). Furthermore, the microscale thermophoresis experiments (MST) on the interaction of compound 8k with TMV coat protein (TMV CP) showed 8k bound to TMV CP with a dissociation constant of 0.97 mmol/L. Docking studies provided further insights into the interaction of 8k with the Arg90 of TMV CP. Conclusions: Sixteen penta-1,4-diene-3-one oxime ether derivatives containing a quinazolin-4(3H)-one scaffold were designed, synthesized, and their antiviral activities against TMV were evaluated. Antiviral bioassays indicated that some target compounds exhibited remarkable antiviral activities against TMV. Furthermore, through the MST and docking studies, we can speculate that 8k inhibited the virulence of TMV by binding Arg90 in TMV CP. These results indicated that this kind of penta-1,4-diene-3-one oxime ether derivatives containing a quinazolin-4(3H)-one scaffold could be further studied as potential alternative templates in the search for novel antiviral agents.

HPLC of Formula: C6H5NO. Bye, fridends, I hope you can learn more about C6H5NO, If you have any questions, you can browse other blog as well. See you lster.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

In 2019.0 SCIENCE published article about NICOTINIC ACETYLCHOLINE-RECEPTORS; SMOKING-CESSATION; PARTIAL AGONISTS; IN-VIVO; DISCRIMINATIVE STIMULUS; GLOBUS-PALLIDUS; WHOLE-BLOOD; VARENICLINE; ALPHA-4-BETA-2; NEURONS in [Magnus, Christopher J.; Lee, Peter H.; Ramirez, Melissa H.; Sternson, Scott M.] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA; [Bonaventura, Jordi; Gomez, Juan L.; Michaelides, Michael] NIDA, Biobehav Imaging & Mol Neuropsychopharmacol Unit, Intramural Res Program, Baltimore, MD 21224 USA; [Zemla, Roland; Basu, Jayeeta] NYU, Inst Neurosci, 550 1st Ave, New York, NY 10016 USA; [Zemla, Roland] NYU, Sch Med, Med Scientist Training Program, New York, NY 10016 USA; [Hu, Xing; Galvan, Adriana] Emory Univ, Yerkes Natl Primate Res Ctr, Atlanta, GA 30329 USA; [Hu, Xing; Galvan, Adriana] Emory Univ, Dept Neurol, Atlanta, GA 30329 USA; [Basu, Jayeeta] NYU, Dept Neurosci & Physiol, Langone Med Ctr, 550 1st Ave, New York, NY 10016 USA; [Michaelides, Michael] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA in 2019.0, Cited 79.0. The Name is 3-Pyridinecarboxaldehyde. Through research, I have a further understanding and discovery of 500-22-1. Name: 3-Pyridinecarboxaldehyde

Chemogenetics enables noninvasive chemical control over cell populations in behaving animals. However, existing small-molecule agonists show insufficient potency or selectivity. There is also a need for chemogenetic systems compatible with both research and human therapeutic applications. We developed a new ion channel-based platform for cell activation and silencing that is controlled by low doses of the smoking cessation drug varenicline. We then synthesized subnanomolar-potency agonists, called uPSEMs, with high selectivity for the chemogenetic receptors. uPSEMs and their receptors were characterized in brains of mice and a rhesus monkey by in vivo electrophysiology, calcium imaging, positron emission tomography, behavioral efficacy testing, and receptor counterscreening. This platform of receptors and selective ultrapotent agonists enables potential research and clinical applications of chemogenetics.

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Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

I found the field of Chemistry very interesting. Saw the article Anti-selective direct asymmetric Mannich reaction catalyzed by protease published in 2019.0. Quality Control of 3-Pyridinecarboxaldehyde, Reprint Addresses He, YH; Guan, Z (corresponding author), Southwest Univ, Sch Chem & Chem Engn, Key Lab Appl Chem Chongqing Municipal, Chongqing 400715, Peoples R China.. The CAS is 500-22-1. Through research, I have a further understanding and discovery of 3-Pyridinecarboxaldehyde

The anti-selective direct asymmetric Mannich reaction of (hetero) aromatic aldehydes, 4-anisidine and O-protected hydroxyacetones for the synthesis of stereodefined anti-beta-amino-alpha-hydroxycarbonyl compounds was developed. Protease type XIV from Streptomyces griseus (SGP) was used as a biocatalyst in 1,4-dioxane/phosphate buffer under mild reaction conditions. The excellent diastereoselectivities of up to >99:1 (anti/syn) and good enantioselectivities of up to 90% ee were achieved. This method provides a more sustainable complement to chemically catalyzed anti-selective direct asymmetric Mannich reactions. (C) 2019 Elsevier Ltd. All rights reserved.

Quality Control of 3-Pyridinecarboxaldehyde. Bye, fridends, I hope you can learn more about C6H5NO, If you have any questions, you can browse other blog as well. See you lster.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

HPLC of Formula: C6H5NO. I found the field of Chemistry very interesting. Saw the article Replacement of an Indole Scaffold Targeting Human 15-Lipoxygenase-1 Using Combinatorial Chemistry published in 2019.0, Reprint Addresses Hirsch, AKH (corresponding author), Univ Groningen, Stratingh Inst Chem, Nijenborgh 7, NL-9747 AG Groningen, Netherlands.; Dekker, FJ (corresponding author), Univ Groningen, GRIP, Chem & Pharmaceut Biol, Antonius Deusinglaan 1, NL-9713 AV Groningen, Netherlands.; Hirsch, AKH (corresponding author), Helmholtz Ctr Infect Res HZI, Helmholtz Inst Pharmaceut Res Saarland HIPS, Dept Drug Design & Optimizat, Campus Bldg E8-1, DE-66123 Saarbrucken, Germany.; Hirsch, AKH (corresponding author), Saarland Univ, Dept Pharm, DE-66123 Saarbrucken, Germany.. The CAS is 500-22-1. Through research, I have a further understanding and discovery of 3-Pyridinecarboxaldehyde.

Human 15-lipoxygenase-1 (15-LOX-1) belongs to the class of lipoxygenases, which catalyze oxygenation of polyunsaturated fatty acids, such as arachidonic and linoleic acid. Recent studies have shown that 15-LOX-1 plays an important role in physiological processes linked to several diseases such as airway inflammation disease, coronary artery disease, and several types of cancer such as rectal, colon, breast and prostate cancer. In this study, we aimed to extend the structural diversity of 15-LOX-1 inhibitors, starting from the recently identified indolyl core. In order to find new scaffolds, we employed a combinatorial approach using various aromatic aldehydes and an aliphatic hydrazide tail. This scaffold-hopping study resulted in the identification of the 3-pyridylring as a suitable replacement of the indolyl core with an inhibitory activity in the micromolar range (IC50=16 +/- 6m) and a rapid and efficient structure-activity relationship investigation.

HPLC of Formula: C6H5NO. Bye, fridends, I hope you can learn more about C6H5NO, If you have any questions, you can browse other blog as well. See you lster.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

Authors Abdelkafi, H; Michau, A; Pons, V; Ngadjeua, F; Clerget, A; Ouarab, LA; Buisson, DA; Montoir, D; Caramelle, L; Gillet, D; Barbier, J; Cintrat, JC in AMER CHEMICAL SOC published article about PROTECTS; TRAFFICKING; ENANTIOMERS; INHIBITION in [Abdelkafi, Hajer; Michau, Aurelien; Ngadjeua, Flora; Clerget, Alexandra; Caramelle, Lucie; Gillet, Daniel; Barbier, Julien] Univ Paris Saclay, Dept Medicaments & Technol Sante DMTS, SIMoS, CEA,INRAE, F-91191 Gif Sur Yvette, France; [Pons, Valerie; Ouarab, Lilia Ait; Buisson, David-Alexandre; Montoir, David; Cintrat, Jean-Christophe] Univ Paris Saclay, Dept Medicaments & Technol Sante DMTS, SCBM, CEA,INRAE, F-91191 Gif Sur Yvette, France in 2020.0, Cited 23.0. Quality Control of 3-Pyridinecarboxaldehyde. The Name is 3-Pyridinecarboxaldehyde. Through research, I have a further understanding and discovery of 500-22-1

High-throughput screening has shown that Retro-1 inhibits ricin and Shiga toxins by diminishing their intracellular trafficking via the retrograde route, from early endosomes to the Golgi apparatus. To improve the activity of Retro-1, a structure-activity relationship (SAR) study was undertaken and yielded an analogue with a roughly 70-fold better half-maximal effective concentration (EC50) against Shiga toxin cytotoxicity measured in a cell protein synthesis assay.

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Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

An article Structure-based exploration and pharmacological evaluation of N-substituted piperidin-4-yl-methanamine CXCR4 chemokine receptor antagonists WOS:000456762500044 published article about FRAGMENT-LIKE LIGANDS; HISTAMINE H-4; MOLECULAR INTERACTION; CRYSTAL-STRUCTURE; HIV ENTRY; IN-VITRO; DISCOVERY; BINDING; INHIBITION; DOCKING in [Adlere, I.; de Esch, I. J. P.; Leurs, R.] Griffin Discoveries BV, Amsterdam, Netherlands; [Sun, S.; Zarca, A.; Roumen, L.; Gozelle, M.; Arimont, M.; Bebelman, J. P.; Smit, M. J.; Vischer, H. F.; Wijtmans, M.; de Graaf, C.; de Esch, I. J. P.; Leurs, R.] Vrije Univ Amsterdam, Fac Sci, Amsterdam Inst Mol Med & Syst, Div Med Chem, De Boelelaan 1108, NL-1081 HZ Amsterdam, Netherlands; [Gozelle, M.] Gazi Univ, Fac Pharm, Dept Pharmaceut Chem, TR-06560 Ankara, Turkey; [Viciano, C. Perpina; Hoffmann, C.] Friedrich Schiller Univ Jena, Univ Hosp Jena, Ctr Mol Biomed, Inst Mol Cell Biol, Hans Knoll Str 2, D-07745 Jena, Germany; [Viciano, C. Perpina; Hoffmann, C.] Univ Wurzburg, Inst Pharmacol & Toxicol, Versbacher Str 9, D-97078 Wurzburg, Germany; [Caspar, B.; Briddon, S. J.; Hill, S. J.] Univ Nottingham, Sch Life Sci, Div Pharmacol Physiol & Neurosci, Nottingham NG7 2UH, England; [Caspar, B.; Briddon, S. J.; Hill, S. J.] Univ Nottingham, Sch Life Sci, Ctr Membrane Prot & Receptors COMPARE, Nottingham NG7 2UH, England in 2019.0, Cited 80.0. Computed Properties of C6H5NO. The Name is 3-Pyridinecarboxaldehyde. Through research, I have a further understanding and discovery of 500-22-1

Using the available structural information of the chemokine receptor CXCR4, we present hit finding and hit exploration studies that make use of virtual fragment screening, design, synthesis and structure activity relationship (SAR) studies. Fragment 2 was identified as virtual screening hit and used as a starting point for the exploration of 31 N-substituted piperidin-4-yl-methanamine derivatives to investigate and improve the interactions with the CXCR4 binding site. Additionally, subtle structural ligand changes lead to distinct interactions with CXCR4 resulting in a full to partial displacement of CXCL12 binding and competitive and/or non-competitive antagonism. Three-dimensional quantitative structure-activity relationship (3D-QSAR) and binding model studies were used to identify important hydrophobic interactions that determine binding affinity and indicate key ligand-receptor interactions. (C) 2018 Elsevier Masson SAS. All rights reserved.

Computed Properties of C6H5NO. Welcome to talk about 500-22-1, If you have any questions, you can contact Adlere, I; Sun, S; Zarca, A; Roumen, L; Gozelle, M; Viciano, CP; Caspar, B; Arimont, M; Bebelman, JP; Briddon, SJ; Hoffmann, C; Hill, SJ; Smit, MJ; Vischer, HF; Wijtmans, M; de Graaf, C; de Esch, IJP; Leurs, R or send Email.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem

An article Design, synthesis, and biological evaluation of 5-((8-methoxy-2-methylquinolin-4-yl)amino)-1H-indole-2-carbohydrazide derivatives as novel Nur77 modulators WOS:000573916100021 published article about CELL-DEATH; ENDOPLASMIC-RETICULUM; DUAL ROLES; APOPTOSIS; EXPRESSION; STRESS; UBIQUITINATION; METABOLISM; ACTIVATION; INDUCTION in [Li, Baicun; Yao, Jie; Guo, Kaiqiang; He, Fengming; Chen, Kun; Lin, Zongxin; Liu, Shunzhi; Huang, Jiangang; Wu, Qiaoqiong; Fang, Meijuan; Zeng, Jinzhang; Wu, Zhen] Xiamen Univ, Sch Pharmaceut Sci, Fujian Prov Key Lab Innovat Drug Target Res, Xiamen 361102, Peoples R China; [Li, Baicun; Yao, Jie; Guo, Kaiqiang; He, Fengming; Chen, Kun; Lin, Zongxin; Liu, Shunzhi; Huang, Jiangang; Wu, Qiaoqiong; Fang, Meijuan; Zeng, Jinzhang; Wu, Zhen] Xiamen Univ, Sch Pharmaceut Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China; [Li, Baicun] Chinese Acad Med Sci, Inst Basic Med Sci, Dept Physiol, State Key Lab Med Mol Biol, Beijing 100005, Peoples R China; [Li, Baicun] Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China in 2020.0, Cited 74.0. Safety of 3-Pyridinecarboxaldehyde. The Name is 3-Pyridinecarboxaldehyde. Through research, I have a further understanding and discovery of 500-22-1

Nur77 is a potential target for the treatment of cancer such as HCC. Herein, we detailed the discovery of a novel series of 5-((8-methoxy-2-methylquinolin-4-yl)amino)-1H-indole-2-carbohydrazide derivatives as potential Nur77 modulators. The studies of antiproliferative activity and Nur77-binding affinity of target compounds resulted in the discovery of a lead candidate (10g), which was a good Nur77 binder (K-D = 3.58 +/- 0.16 mu M) with a broad-spectrum antiproliferative activity against all tested hepatoma cells (IC50 < 2.0 mu M) and was low toxic to normal LO2 cells. 10g could up-regulate Nur77 expression and mediate sub-cellular localization of Nur77 to induce apoptosis in hepatocellular carcinoma cell lines, which relied on 10g inducing Nur77-dependent autophagy and endoplasmic reticulum stress as the upstream of apoptosis. Moreover, the in vivo assays verified that 10g significantly inhibited xenograft tumor growth. These results indicate that 10g has the potential to be developed as a novel Nur77-targeting anti-hepatoma drug. (C) 2020 Elsevier Masson SAS. All rights reserved. Safety of 3-Pyridinecarboxaldehyde. Welcome to talk about 500-22-1, If you have any questions, you can contact Li, BC; Yao, J; Guo, KQ; He, FM; Chen, K; Lin, ZX; Liu, SZ; Huang, JG; Wu, QQ; Fang, MJ; Zeng, JZ; Wu, Z or send Email.

Reference:
Pyridine – Wikipedia,
,Pyridine | C5H5N – PubChem