The Mechanism of Orotidine 5′-Monophosphate Decarboxylase: Catalysis by Destabilization of the Substrate was written by Feng, Wan Yong;Austin, Travis J.;Chew, Frank;Gronert, Scott;Wu, Weiming. And the article was included in Biochemistry in 2000.Name: 1-Methyl-6-oxo-1,6-dihydropyridine-2-carboxylic acid This article mentions the following:
The mechanism of orotidine 5′-monophosphate decarboxylase (OMP decarboxylase, ODCase) was studied using the decarboxylation of orotic acid analogs as a model system. The rate of decarboxylation of 1,3-dimethylorotic acid and its analogs as well as the stability of their corresponding carbanion intermediates was determined The results have shown that the stability of the carbanion intermediate is not a critical factor in the rate of decarboxylation. On the other hand, the reaction rate is largely dependent on the equilibrium constant for the formation of a zwitterion. Based on these results, we have proposed a new mechanism in which ODCase catalyzes the decarboxylation of OMP by binding the substrate in a zwitterionic form and providing a destabilizing environment for the carboxylate group of OMP. In the experiment, the researchers used many compounds, for example, 1-Methyl-6-oxo-1,6-dihydropyridine-2-carboxylic acid (cas: 59864-31-2Name: 1-Methyl-6-oxo-1,6-dihydropyridine-2-carboxylic acid).
1-Methyl-6-oxo-1,6-dihydropyridine-2-carboxylic acid (cas: 59864-31-2) belongs to pyridine derivatives. In contrast to benzene, Pyridine’s electron density is not evenly distributed over the ring, reflecting the negative inductive effect of the nitrogen atom. Many analogues of pyridine are known where N is replaced by other heteroatoms . Substitution of one C–H in pyridine with a second N gives rise to the diazine heterocycles (C4H4N2), with the names pyridazine, pyrimidine, and pyrazine.Name: 1-Methyl-6-oxo-1,6-dihydropyridine-2-carboxylic acid