Isothermal titration calorimetry investigation of the interactions between vitamin B6-derived hydrazones and bovine and human serum albumin was written by Migliore, Rossella;Zavalishin, Maksim N.;Gamov, George A.;Usacheva, Tatiana R.;Sharnin, Valentin A.;Grasso, Giuseppa I.;Sgarlata, Carmelo. And the article was included in Journal of Thermal Analysis and Calorimetry in 2022.Name: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:
The binding of low mol. weight compounds with the transport proteins of blood is an essential step of their delivery into living cells and thus the accurate investigation of the interactions occurring in solution at physiol. conditions is crucial for the development of efficient biol. active mols. In this work, we report on the complex species, stability constants and thermodn. parameters for the binding reactions of hydrazones derived from pyridoxal-5鈥?phosphate (PLP) with bovine and human serum albumin (BSA and HSA) in neutral aqueous solution The study has been carried out using isothermal titration calorimetry which allowed to directly obtain both binding constant and enthalpy change values for the systems investigated. The thermodn. characterization in solution revealed that the PLP-hydrazone derivatives are able to effectively interact with both bovine and human serum albumin and enabled the determination of the driving forces for the mol. recognition process. The formation of the 1:1 complex was found to be always enthalpy favored and driven due to the insertion of the hydrazone moieties into the hydrophobic pockets of BSA or HSA. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Name: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine’s the lone pair does not contribute to the aromatic system but importantly influences the chemical properties of pyridine, as it easily supports bond formation via an electrophilic attack. Many analogues of pyridine are known where N is replaced by other heteroatoms . Substitution of one C鈥揌 in pyridine with a second N gives rise to the diazine heterocycles (C4H4N2), with the names pyridazine, pyrimidine, and pyrazine.Name: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate